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Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the <t>CellTOX</t> Green <t>Cytotoxicity</t> Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.
Celltox Green Cytotoxicity Assay Kit G8710, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltox+tm+green+cytotoxicity/celltox+green+cytotoxicity+assay+kit+g8710/pmc10982647-56-12-18
Average 90 stars, based on 1 article reviews
celltox green cytotoxicity assay kit g8710 - by Bioz Stars, 2026-09
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Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the <t>CellTOX</t> Green <t>Cytotoxicity</t> Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.
Celltox Green Cytotoxicity Assay, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltox+tm+green+cytotoxicity/celltox+green+cytotoxicity+assay/10__3390_slash_jcto2010001-191-0-30
Average 90 stars, based on 1 article reviews
celltox green cytotoxicity assay - by Bioz Stars, 2026-09
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Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the <t>CellTOX</t> Green <t>Cytotoxicity</t> Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.
Celltox Green Cytotoxicity Assay G874, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltox+tm+green+cytotoxicity/celltox+green+cytotoxicity+assay+g874/pmc07915079-152-22-26
Average 90 stars, based on 1 article reviews
celltox green cytotoxicity assay g874 - by Bioz Stars, 2026-09
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Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the <t>CellTOX</t> Green <t>Cytotoxicity</t> Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.
Celltox Green Cytotoxicity Assay Tm375, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltox+tm+green+cytotoxicity/celltox+green+cytotoxicity+assay+tm375/pm27229448-289-4-10
Average 90 stars, based on 1 article reviews
celltox green cytotoxicity assay tm375 - by Bioz Stars, 2026-09
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Image Search Results


Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the CellTOX Green Cytotoxicity Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.

Journal: Cancer Research

Article Title: PRMT1 Sustains De Novo Fatty Acid Synthesis by Methylating PHGDH to Drive Chemoresistance in Triple-Negative Breast Cancer

doi: 10.1158/0008-5472.CAN-23-2266

Figure Lengend Snippet: Enhanced arginine methylation of glycolytic enzymes and activation of serine synthetic pathway in the chemoresistant breast cancer cell lines. A and B, Magnitudes of cell death were assessed using the CellTOX Green Cytotoxicity Assay kit. Chemosensitivity to doxorubicin ( A ) and paclitaxel ( B ). Mean ± SE ( n = 4) *, P < 0.05, versus MCF7 (yellow), an ER-positive breast cancer cell line. Comparisons among all four groups were performed using one-way ANOVA with Fisher LSD test. C, Left, Western blot analyses showing basal expression of enzymes in glycolysis, serine-synthesizing system, and trans-sulfuration pathways in human breast cancer-derived cell lines. Right, each enzyme was mapped to a metabolic pathway. The dataset is representative of at least three independent experiments. D, Protocols to generate Ptx-resistant MDA-MB-231 cells, in which the cells were transplanted into nude mice. E, Western blot analyses showing expression of enzymes in glycolysis, the serine-synthesizing system, and the trans-sulfuration pathway in three different cell lines of Ptx-resistant (Ptx-R) MDA-MB-231 (#1–3). F, Effects of increasing concentration of Ptx on cell death, which was assessed using the CellTOX Green Cytotoxicity Assay kit. *, P < 0.05, compared with parenteral cell death. Data indicate mean ± SE ( n = 6). *, P < 0.05 versus parental cells. Differences were tested using two-way ANOVA with Fisher LSD test. G and H, Effects of sgPRMT1 on the cell death of parental ( G ) and Ptx-R ( H ) MDA-MB-231 cells. * and †, P < 0.05, two-way ANOVA with Fisher least significant difference test. Data indicate mean ± SE ( n = 4). n.s, not significant.

Article Snippet: Using several different human-derived cell lines, cell viability was assessed using the CellTOX Green Cytotoxicity Assay Kit (G8710; Promega; refs. ) according to the manufacturer's instructions.

Techniques: Methylation, Activation Assay, CellTox Assay, Cytotoxicity Assay, Western Blot, Expressing, Derivative Assay, Concentration Assay